AP-100 Online biochemistry analyzer
AP-100 Online biochemistry analyzer makes online sampling and feeding concentration control more easier.
AP-100 Online biochemistry analyzer
Full automation, good accuracy and precision!
Multiple bioreactors can be sampled, analyzed, feeding controlled online!
By integrating the online module and upgrading the optimization control software, Sieman has launched AP-100 Automated Online Biochemistry Analyzer, a biochemical analysis system for online sampling and control of feed concentration. It is no longer difficult to make biological fermentation on-line sampling and feeding control.
The AP-100 analyzer can online analysis and feed control of biochemical parameters including：
Glucose, Glutamine, Lactate, Lysine, Methanol/Ethanol, Glycerin, Sucrose, Xylose, Glutamate, NH4+, K+, Na+, Ca++, pH, Osm, etc.
Reduce the manual sampling operation of the fermentation process, and realize the fine feed control of biological fermentation and culture process.
After real time automatic sampling, the bioreactor can be automatically fed in real time.
The following is an application image of the AP-100 online biochemistry analyzer:
This is a video of the operation of the AP-100 online analyzer:
AP-100 Online Biochemistry Analyzer Application Scope
- Provide reliable and stable process analysis data for the development and production of recombinant proteins, and control the feeding concentration
- Monitor nutrient depletion of the medium and accumulation of metabolic wastes during cell culture
- Glucose metabolism rate was detected in adherent culture to indirectly reflect cell growth
- Accurately control the timing of medium replacement to ensure the reproducibility of experimental results
- Determine the key control points of the process
AP-100 Online Biochemistry Analyzer Features
- It can collect biochemical (carbon and nitrogen source) parameters in the process of biological culture in near real time, so as to realize multi-perspective monitoring and analysis of the whole biological culture process
- The specificity of enzyme electrode method to the substrate ensures that it can be developed from laboratory research and development to large-scale cGMP manufacturing
- Fully closed sterile sampling system is still safe and reliable after thousands of times of verification
- A single instrument can sample four reactors at the same time
- The sampling probe is PG13.5 or ingold interface, suitable for various stainless steel, glass reactors and disposable reactors
- General 4-20mA analog signal output, can be connected to the local automation control system (such as DCS system)
- According to customer requirements, 1~5 projects can be selected to monitor at the same time
- LIS data acquisition and control software can be selected to realize various fed-material culture strategies such as constant concentration, stepwise concentration and time-phased concentration culture
Analysis of Glucose item detection data during fermentation
| Off-line test date g/L | 12.15 | 9.51 | 10.6 | 4.7 | 8.39 | 9.97 | 9.58 | 3.99 | 8.11 |
| On-line test date g/L | 12.29 | 9.85 | 10.51 | 4.6 | 8.28 | 10.66 | 9.82 | 4.1 | 8 |
AP-100 Online Biochemistry Analyzer Specifications
Item name |
Optimum analysis range |
Precision，CV |
Remark |
| Glucose | 0.3~9 g/L | 2% | Resolution 0.1 g/L |
| 0.05~1 g/L | 2% | Resolution 0.01 g/L | |
| Lactate | 0.03~2 g/L | 2% | |
| Glutamate | 0.03~1 g/L | 2% | |
| Glutamine | 0.03~1 g/L | 2% | |
| Lysine | 0.03~1 g/L | 2% | |
| Ethanol | 0.03~1 g/L | 2% | |
| Methanol | 0.03~0.5 g/L | 2% | |
| Glycerol | 0.03~1 g/L | 2% | |
| Xylose | 0.03~1 g/L | 2% | |
| pH | 6.00-8.00 | 0.01 | Automatic Calibration |
| NH4+ | 2.0~30.0 mmol/L | 2% | |
| Na+ | 60.0~200.0 mmol/L | 2% | |
| K+ | 0.5~15.0 mmol/L | 2% | |
| Ca2+ | 0.1~5.0 mmol/L | 2% | |
| Optional dilution module to achieve 10-30 times dilution of online sampling samples. | |||
| Single sampling Volume | ＜5mL | ||
| Sampling Interval | ＞15min | ||
| Sampling probe Interface | PG13.5 or ingold thread | ||
| Sampling probe Length | 120/225/310/325mm | ||
| Filter membrane pore size | 0.2-20um | ||
Through the automatic control of ceramic membrane sampling rod sampling(Choose not to install ceramic membrane, direct sampling, also will not cause contamination), accurate detection on the analyzer, the results are fed back to the feed pump, automatic calculation of feed speed and volume.
AP-100 is used for a range of concentration controls, such as controlling glucose concentration in parallel cell culture bioreactors, glutamine concentration in hybridoma cells, glycerol concentration in yeast culture, and so on. The AP-100 analyzer can online sampling up to four bioreactors simultaneously, providing the customer more precise process control.
Sieman LIS software Interface Layout Overview
