M-100 Biosensor analyzer
M-100 Biosensor analyzer is especially suitable for the detection of the corresponding metabolic substance concentration during cell culture.
M-100 Biosensor Analyzer Introduction
The sample testing is fast, stable and cost-effective, and the sample biochemical parameters could be automatically calibrated & tested.
The M-100 biosensor analyzer maintaining the same performance after continuous use for many years, providing customers with easy parameter testing services.
M-100 biosensor analyzer is especially suitable for the fermentation industry, which requires frequent detection of the corresponding substance concentration.
Application Fields:
Biopharmaceutical process parameter analysis, Biological fermentation metabolism parameter analysis, Cell culture flask parameter analysis, Food manufacturing process parameter analysis, Laboratory research, etc.
Test Parameters:
Glucose, Lactate, Glutamate, Lysine, Ethanol, Methanol, Glutamine, Glycerin, Xylose, Sucrose, etc.
The Principle of M-100 Biosensor Analyzer
M-100 is available for detecting the concentration of the sample by use of the specially designed bio-oxidase membrane electrochemical sensor.
M-100 Biosensor analyzer could automatically sucks the sample and put the sample into the test area. The test substance contained in the sample will undergo an enzymatic hydrolysis reaction under the catalysis of an immobilized bio-oxidase to generate hydrogen peroxide. The concentration of the test substance is calculated by the hydrogen peroxide concentration that is generated in the testing process of hydrogen peroxide electrode.
The device will automatically calibrate a standard solution with a known concentration, and the voltage of the standard solution is a measure of the concentration of the test substance. The unknown concentration can be obtained through the comparison with the voltage signal of the standard solution. And the electrodes of M-100 biosensor analyzer will be automatically cleaned by the buffer after each measurement is completed, then the next test can be performed.
M-100 Biosensor Analyzer Features
- Faster testing speed, the reaction time only needs 20 seconds;
- Independent r&d of new immobilized enzyme membrane technology, longer life, lower sample testing cost;
- Automated calibration and sample testing, reduce human error;
- Electrochemical detection, strong anti-interference ability, it is not affected by the background color of the fermentation liquid;
- Multi-function menu, man-machine interactive operation, multi-user login to meet audit trail requirements;
- Supports 3Q authentication;
- Freely set the automatic repeated testing times to provide more reliable sample data;
- All sample data and calibration data can be exported by one click;
- With 15 sample loading positions, the device can automatically complete the test of selected samples in order.
- After placing the standard solution and samples, click the “Test” icon in the main menu interface, then select the cup number where the sample is located. Click “TEST”, The instrument will automatically perform calibration and complete all sample tests.
M-100 Biosensor Analyzer Specifications
| Test principle | Enzyme electrode method |
| Electrode | Rod electrode |
| Measuring range | Glucose(G1001): 0.05~1 g/L
Glucose(C1001): 0.3~9 g/L Lactate: 0.03~2 g/L Glutamate: 0.03~1 g/L Lysine: 0.03~1 g/L Methanol: 0.03~0.5 g/L Ethanol: 0.03~1 g/L Xylose：0.03~1 g/L Glycerol：0.03~1 g/L Glutamine：0.03~1 g/L |
| Resolution | 0.01g/L |
| Precision (CV%) | <2% |
| Enzyme membrane life | Glucose: ≤30 days, Lactate: ≤30 days, Glutamate: ≤30 days,
Lysine: ≤30 days, Ethanol / methanol: ≤10 days, Xylose: ≤15days, Glycerol: ≤15days, Glutamine：≤15 days |
| Ambient temperature | 10~35°C |
| Relative humidity | ≤85% (no condensation water) |
| External dimension | 480×320×210 mm |
| Power supply | 110V or 220V, 50Hz, 60Hz, separate three-hole socket with good grounding |
| Power | 100VA |
| Weight | 9kg |
| Screen | 8-inch color touch screen |
*Automatic sampling and testing
Comparison of M-100 & HPLC Test Results
Test example: Glucose
Detection range: Glucose: 0-9g/L（0-50mmol/L）
Test example: Lysine
1. Pre-treatment: taking advantage of the property that lysine hydrochloride is easily soluble in water (40g/ml,35℃), 1.00g of 98% commercially available feed grade L-lysine hydrochloride sample was taken and fully dissolved, with a constant volume of 100mL, and then tested on the device
2. Results
| Brand | 1 | 2 | 3 | 4 |
| M-100 analyzer, % | 98.88 | 98.92 | 98.85 | 98.99 |
| HPLC，% | 98.63 | 98.89 | 98.52 | 98.68 |
| Perchloric acid titration, % | 98.96 | 98.82 | 99.11 | 98.71 |
M-100 Biosensor Analyzer Diagrammatic
