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M-100 Biosensor analyzer

M-100 Biosensor analyzer is especially suitable for the detection of the corresponding metabolic substance concentration during cell culture.

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M-100 Biosensor Analyzer Introduction

The biochemical parameters of the samples could be automatically calibrated & tested. The sample testing speed is fast, result accuracy. It has a stable analytical process and good cost effectiveness.

The M-100 biosensor analyzer has maintained the same performance after years of continuous use, providing customers with more easier biochemical parameters testing service.

M-100 biosensor analyzer is especially suitable for the bio-fermentation industry, which requires frequent detection of the corresponding substance concentration.

 

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Application Fields:

Biopharmaceutical bioprocess parameter analysis,

Biological fermentation metabolism parameter analysis,

Cell culture flask medium parameter analysis,

Food manufacturing process parameter analysis,

Laboratory research in research institutions or specialized universities, etc.

 

Test Parameters:

Glucose,  Lactate,  Glutamate,  Lysine,  Ethanol,  Methanol,  Glutamine,  Glycerin,  Xylose,  Sucrose,  etc.

 

 

The Principle of M-100 Biosensor Analyzer

Principle

The M-100 analyzer uses a specially designed biological oxidase membrane electrochemical sensor to detect the concentration of the sample..

M-100 Biosensor analyzer could automatically sucks the sample and put the sample into the test area. The test substance contained in the sample will undergo an enzymatic hydrolysis reaction under the catalysis of an immobilized bio-oxidase to generate hydrogen peroxide. The concentration of the test substance is calculated by the hydrogen peroxide concentration that is generated in the testing process of hydrogen peroxide electrode.

The device will automatically calibrate standard liquids at known concentrations, and the voltage of the standard liquid is a measure of the concentration of the test substance. The unknown concentration can be obtained through the comparison with the voltage signal of the standard liquid. And the electrodes of M-100 biosensor analyzer will be automatically cleaned by the buffer after each measurement is completed, then the next test can be performed.

 

M-100 Biosensor Analyzer Features

  • Faster testing speed, the reaction time only needs 20 seconds;
  • Independent r&d of new immobilized enzyme membrane technology, longer life, lower sample testing cost;
  • Automated calibration and sample testing, reduce human error;
  • Electrochemical detection, strong anti-interference ability, it is not affected by the background color of the fermentation liquid;
  • Multi-function menu, man-machine interactive operation, multi-user login to meet audit trail requirements;
  • Supports 3Q authentication;
  • Freely set the automatic repeated testing times to provide more reliable sample data;
  • All sample data and calibration data can be exported by one click;
  • With 15 sample loading positions, the device can automatically complete the test of selected samples in order.
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  • After placing the standard liquid and samples, click the “Test” icon in the main menu interface, then select the cup number where the sample is located. Click “TEST”, The instrument will automatically perform calibration and complete all sample tests.

 

 

M-100 Biosensor Analyzer Specifications

Test principle Enzyme electrode method
Electrode Rod electrode
Measuring range Glucose(G1001): 0.05~g/L

Glucose(C1001): 0.3~9 g/L

Lactate: 0.03~2 g/L

Glutamate: 0.03~1 g/L 

Lysine: 0.03~1 g/L

Methanol: 0.03~0.5 g/L

Ethanol: 0.03~1 g/L

Xylose:0.03~1 g/L

Glycerol:0.03~1 g/L

Glutamine:0.03~1 g/L

Resolution 0.01g/L
Precision (CV%) <2%
Enzyme membrane life Glucose: 30 days, Lactate: 30 days, Glutamate: 30 days,

Lysine: 30 days, Ethanol / methanol: 10 days, Xylose: ≤15days,

Glycerol: ≤15days, Glutamine:≤15 days

Ambient temperature 10~35°C
Relative humidity ≤85% (no condensation water)
External dimension 480×320×210 mm
Power supply 110V or 220V, 50Hz, 60Hz, separate three-hole socket with good grounding
Power 100VA
Weight 9kg
Screen 8-inch color touch screen

*Automatic sampling and testing

 

Comparison of M-100 & HPLC Test Results

 

Test example: Glucose
Detection range: Glucose: 0-9g/L(0-50mmol/L)

M-100 Automated Biosensor Analyzer 02

Test example: Lysine
1. Pre-treatment: taking advantage of the property that lysine hydrochloride is easily soluble in water (40g/ml,35℃), 1.00g of 98% commercially available feed grade L-lysine hydrochloride sample was taken and fully dissolved, with a constant volume of 100mL, and then tested on the device
2. Results

Brand 1 2 3 4
M-100 analyzer, % 98.88 98.92 98.85 98.99
HPLC,% 98.63 98.89 98.52 98.68
Perchloric acid titration, % 98.96 98.82 99.11 98.71

 

M-100 Biosensor Analyzer Diagrammatic

 

M-100 Automated Biosensor Analyzer04

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