M-2000 Bioprocess biochemistry analyzer
M-2000 Bioprocess biochemistry analyzer can process 170 biochemical parameter tests per hour, support 96-well plates and 20 EP tubes
M-2000 Bioprocess Biochemistry Analyzer Introduction
M-2000 Bioprocess biochemistry analyzer is an intelligent high throughput & automated analysis equipment, with economical and faster testing speed, easier operation, high precision.
Different loading modules, including 96-well plates and 20 EP tubes, can be automatically identified and 170 samples of biochemical parameters can be tested per hour.
M-2000 Bioprocess biochemistry analyzer using integrated kits, plug and play, fully automated calibration.
Test menu:
Glucose, Sucrose, Lactate, Glutamine, Glutamate, Lysine, Ethanol, Methanol, Glycerol, Xylose, pH, NH4+, K+, Na+, Ca2+, Calculate Osm, etc.
Application field:
CDMO monoclonal antibody development, Analysis of the production process of recombinant protein, Analysis of biological culture process of biopharmaceutical, Analysis of biochemical and metabolic parameters in cell screening, Process analysis of parallel bioreactor in biological culture, etc.
M-2000 Bioprocess Biochemistry Analyzer diagrammatic
M-2000 Bioprocess Biochemistry Analyzer Principle
Hydrogen peroxide electrode: An oxidase corresponding to the test substrate is fixed in the enzyme membrane, and the substrate is catalyzed to produce hydrogen peroxide. The hydrogen peroxide is measured by a hydrogen peroxide electrode, and the concentration of the test substrate is calculated from the hydrogen peroxide concentration.
Ion-selective electrode（ISE） is a type of electrochemical sensor that can convert the changes in the activity of the ions in the solution into changes in the electrode potential, and the logarithm of the ion activity in the solution has a linear relationship with the electrode potential. “Ion-selective electrode” means that the sensor is only sensitive to specific ions. For example, the ammonium electrode is only sensitive to ammonium ions in the solution, but not sensitive to potassium and other ions.
After reagent and sample are placed, the instrument will automatically finish calibration, click the “Test” icon in the main menu interface, and then click the cup where the sample is located, start-up test, the instrument will automatically complete the test.
M-2000 Bioprocess biochemistry analyzer Features
- Faster testing speed. Biochemical item can process 170 samples per hour;
- It supports 96-well plates and 20 EP tubes, automated system identification;
- Patented flow technology, buffer and cleaning solution are connected by quick connector;
- Automatic injection, automatic calibration, consumable control function;
- 12.1 inch high-definition capacitive touch color screen, 90° adjustable, instrument built-in automatic switch LED observation light;
- Better testing performance than liquid enzyme colorimetric technology (no interference by background color), CV≤2%;
- Low sample volume demand design, test all items minimum volume requirement 300uL;
- Daily automatic maintenance function, built-in scanning module, support consumable information update;
- Multi-function menu, multi-user login to meet the authentication requirements, MCU main control software can be upgraded;
- It can be used in the cultivation process analysis of mammalian cells, bacteria, fungi, yeast, algae and so on. It supports the expansion of modules and can measure 8 enzyme items +5 ion items at the same time；
- Automatic dilution optional, independent dilution cell, automatic detection, real unattended analysis;
- Support 3Q certification;
- The number of automated repeated tests can be set to provide more reliable testing data;
- Storage sample data ≤10000, calibration data ≤1000, automatically overwrites previous data;
- It supports RS232 and RJ45 communication interfaces, support HP laser printer, thermal printer and needle printer;
- Test data, calibration data can be exported by U disk.
Sample treatment is divided into two scenarios.
1) Cell culture medium: Since the cell culture medium itself has sufficient ionic strength, it can be directly tested without pre-treatment.
2) Aqueous solutions that cannot meet the requirements of ionic strength:
a: First prepare a solution of 16g/L sodium chloride (which cannot be replaced by other substances) for use.
b: Mix the sample to be tested with the sodium chloride solution prepared in step a at a volume ratio of 1:1 to obtain a mixture with sufficient ionic strength
sample solution.
c: Multiply the result obtained by machine test of the sample solution by 2 to obtain the actual ion concentration of the sample.
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Accuracy of Glucose concentration in cell medium |
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| Number of samples | The average number test results mg/dl | SD | % CV |
| 50 | 68 | 1.36 | 2 |
| 50 | 189.6 | 3.41 | 1.8 |
| 50 | 265.7 | 5.05 | 1.9 |
| 50 | 351.2 | 5.27 | 1.5 |
| 50 | 501.8 | 10.04 | 2 |
| 50 | 619.6 | 12.39 | 2 |
M-2000 Bioprocess Automated Biochemistry Analyzer Specifications
| Test principle | Enzyme electrode method |
| Electrode | Single replaceable electrode |
| Sample holder | 96-well plate or 20 EP cups holder |
| Measuring range | Glucose(Article number: C1001): 0.3~9 g/L
Glucose(Article number: G1001): 0.05~1 g/L Glutamine: 0.03~1 g/L Glycerol: 0.03~1 g/L Xylose: 0.03~1 g/L Lactate: 0.03~2 g/L Glutamate: 0.03~1 g/L Lysine: 0.03~1 g/L Methanol: 0.03~0.5 g/L Ethanol: 0.03~1 g/L The machine with pre-dilution function can expand the above test range by 10 to 30 times. NH4+: 2～30mmol/L, Na+: 60～200mmol/L, K+: 0.5～15mmol/L, Ca2+: 0.1～5mmol/L, pH: 6～8 |
| Resolution | 0.01g/L; 0.01mmol/L |
| Precision (CV%) | <2% |
| Enzyme membrane life | Glucose: ≤30 days, Lactate:≤30days, Glutamate: ≤30 days,
Lysine: ≤30 days, Ethanol / methanol: ≤10 days, Glutamine: ≤15days, Glycerin: ≤15 days, Xylose: ≤15 days, NH4+ electrode: ≥4 months, Na+ electrode: ≥6 months, K+ electrode: ≥6 months, Ca2+ electrode: ≥6 months, pH electrode: ≥4 months |
| Ambient temperature | 10~35°C |
| Relative humidity | ≤85% (no condensation water) |
| External dimension | 600×420×465 mm |
| Power supply | 110V or 220V, 50Hz, 60Hz, separate three-hole socket with good grounding |
| Power | ＜100VA |
| Weight | 48kg |
| Screen | 12.1-inch color touch screen |
*Automatic calibration, injection and sample testing, automatic dilution
