M-900 plus Bioprocess biochemistry analyzer
M-900 plus bioprocess biochemistry analyzer is suitable for metabolic parameters analysis in the cell culture process development.
M-900 plus Bioprocess Biochemistry Analyzer Brief Introduction
M-900 plus Bioprocess Biochemistry analyzer can automatically test biochemical & ionic parameters in samples, up to 6 biochemical parameters and 5 ionic parameters can be selected, automated calibration before sample testing. Equipped with an integrated ion reagent kit, calculates osmotic pressure and can be connected to the Sieman Laboratory Information Management System -LIS software.
M-900 plus Bioprocess Biochemistry analyzer is especially suitable for the cell culture process development.
The accuracy and precision of M-900 plus analyzer are proven to be reliable, and the cost of M-900 plus consumables is low, the test speed of M-900 plus is fast, just take 20s for a single biochemical parameter. All parameters of the sample could be tested within 2 min.
Application Fields:
Biopharmaceutical process parameter analysis, Biological fermentation metabolism parameter analysis, Cell culture flash parameter analysis, Food manufacturing process parameter analysis, Laboratory research, etc.
Test Parameters:
Glucose, Sucrose, Lactate, Glutamine, Glutamate, Lysine, Ethanol, Methanol, Glycerol, Xylose, pH, NH4+, K+, Na+, Ca2+, Osm, etc.
M-900 plus Bioprocess Biochemistry Analyzer Demo Testing Video:
The Principle of M-900 plus Bioprocess Biochemistry Analyzer
M-900 plus analyzer uses new immobilized enzyme membrane electrode and ion selective electrode to detect the concentration of the sample.
Hydrogen peroxide electrode: An oxidase corresponding to the test substrate is fixed in the enzyme membrane, and the substrate is catalyzed to produce hydrogen peroxide. The hydrogen peroxide is measured by a hydrogen peroxide electrode, and the concentration of the test substrate is calculated from the hydrogen peroxide concentration.
Ion-selective electrode（ISE） is a type of electrochemical sensor that can convert the changes in the activity of the ions in the solution into changes in the electrode potential, and the logarithm of the ion activity in the solution has a linear relationship with the electrode potential. “Ion-selective electrode” means that the sensor is only sensitive to specific ions. For example, the ammonium electrode is only sensitive to ammonium ions in the solution, but not sensitive to potassium and other ions.
M-900 plus analyzer automatically sucks samples and puts them into the test area. The test substance contained in the sample generates an electrical signal on a specific electrode. The instrument calculates the concentration of the test object by collecting the electrical signals on the electrodes. Before the test, the instrument automated calibrates the standard solution with a known concentration, and the voltage of the standard solution is a measure of the concentration of the test substance. The unknown concentration can be obtained through the comparison with the voltage signal of the standard solution. All the electrodes will be automatically cleaned by the system buffer after each measurement is completed, and the next test can be performed after cleaning.
M-900 plus Bioprocess Biochemistry Analyzer Features
- With mixing and cleaning system, optional automated dilution
- High-precision Teflon coating sampler
- Excellent pump and valve control system with ultra-high reliability
- Fully automatic calibration to ensure the accuracy of test results
- Only a small volume of samples can be tested at any time
- Reliable performance and quality due to independent core technologies based on more than 10 years of manufacturing experience
- Excellent electromagnetic compatibility performance to ensure the reliability of the device system
- Built-in sample tray with up to 15 sample positions, supporting EP tubes and micro cups
- Visual and intuitive operation interface, 10-inch color touch screen human-computer interaction
- Test results being reviewed, printed and transmitted in real time
- External reagent in low cost and easy operation
Correlation between M-900 plus and HPLC test results
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Accuracy and precision of Glucose concentration in cell medium |
|||
| Number of samples | The average number test results mg/dl | SD | CV % |
| 50 | 68 | 1.36 | 2 |
| 50 | 189.6 | 3.41 | 1.8 |
| 50 | 265.7 | 5.05 | 1.9 |
| 50 | 351.2 | 5.27 | 1.5 |
| 50 | 501.8 | 10.04 | 2 |
| 50 | 619.6 | 12.39 | 2 |
M-900 plus Bioprocess Biochemistry Analyzer Specifications
| Test principle | Enzyme electrode method |
| Electrode | Single replaceable electrode |
| Measuring range | Glucose(Article number: C1001): 0.3~9 g/L
Glucose(Article number: G1001): 0.05~1 g/L Glutamine: 0.03~1 g/L Glycerol: 0.03~1 g/L Xylose: 0.03~1 g/L Lactate: 0.03~2 g/L Glutamate: 0.03~1 g/L Lysine: 0.03~1 g/L Methanol: 0.03~0.5 g/L Ethanol: .0.03~1 g/L Dilution function is optional. The machine with pre-dilution function can expand the above test range by 10 to 30 times. NH4+: 2～30mmol/L, Na+: 60～200mmol/L, K+: 0.5～15mmol/L, Ca2+: 0.1～5mmol/L, pH: 6～8 |
| Resolution | 0.01g/L; 0.01mmol/L |
| Precision (CV%) | <2% |
| Enzyme membrane life | Glucose: ≤30 days, Lactate:≤30days, Glutamate: ≤30 days,
Lysine: ≤30 days, Ethanol / methanol: ≤10 days, Glutamine: ≤15days, Glycerol: ≤15 days, Xylose: ≤15 days, NH4+ electrode: ≥6 months, Na+ electrode: ≥6 months, K+ electrode: ≥6 months, Ca2+ electrode: ≥6 months, pH electrode: ≥6 months |
| Ambient temperature | 10~35°C |
| Relative humidity | ≤85% (no condensation water) |
| External dimension | 660×530×520 mm |
| Power supply | 110V or 220V, 50Hz, 60Hz, separate three-hole socket with good grounding |
| Power | ＜100VA |
| Weight | 35kg |
| Screen | 10-inch color touch screen |
*Automatic sample loading and detection, automatic dilution is optional
M-900 plus Bioprocess Biochemistry Analyzer Diagrammatic
